In TWIST 產品

NGS

Twist獨家設計雙股DNA探針可精準抓取目標序列片段外,亦可同時保留雙股突變資訊。

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[Products]

Twist人類外顯子探針組,精準抓取目標區域並擴增,可提高目標片段的定序深度以降低成本。

① Twist Human Comprehensive Exome Panel (Exome Panel + RefSeq Panel (可單獨購買))

  • 涵蓋人類蛋白質編碼區域高達 99%
  • 廣泛包含 CCDS、RefSeq、GENCODE 資料庫
  • 快速添加不同客製化探針組(Spike-in panels)

NGS comprehensive

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② Twist Human Core Exome Panel

  • 精準抓取人類外顯子核心區域(33Mb)、減少定序量
  • 增加樣本通量降低成本

③ Mitochondrial Panel

  • 涵蓋粒線體 37 個基因(16,659 bp)
  • 快速比對粒線體 DNA 變異疾病

ngs_mitochondrial-panel

④ Mouse Exome Panel

  • 針對UCSC小鼠mm10組裝基因體作為設計範本
  • 精準抓取小鼠外顯子主要核心區域(37.7 Mb)
  • 於20倍深度僅5Gb定序量即可涵蓋95%目標區域
  • 靈敏度高適用於偵測高複雜性資料庫的基因體變異ngs_mouse-exome-panel

      Product list

  • 102035 Twist Mouse Exome, 2 Reactions, Kit
  • 102036 Twist Mouse Exome, 12 Reactions, Kit
  • 102037 Twist Mouse Exome, 96 Reactions, Kit

⑤ Respiratory Virus Research Panel

  • 包含29種常見的人類上呼吸道病毒
  • 有效區分上呼吸道病毒感染與COVID-19病原
  • 多種病毒一網打盡
  • 潛伏期即可偵測

virus

      Product list

  • 103066 Twist Respiratory Virus Research Panel with One Codex software, 2 Reactions, Kit
  • 103067 Twist Respiratory Virus Research Panel with One Codex software, 12 Reactions, Kit
  • 103068 Twist Respiratory Virus Research Panel with One Codex software, 96 Reactions, Kit

⑥ Comprehensive Viral Research Panel

  • 超過一百萬條獨特探針可精準探測 3,153 種病毒基因體
  • 獨家雙股DNA 探針可完整抓取不同病毒基因體資訊
  • 探針具錯配容忍度利於發現新興病毒
  • 涵蓋人類與非人類致病病原
  • One Codex分析報告完整追蹤病毒變異株
  • 可即時產生分析報告及圖表

ngs_comprehensive-viral-research-panel

      Product list

  • 103545 Twist Comprehensive Viral Research Panel with One Codex software, 2 Reactions, Kit
  • 103547 Twist Comprehensive Viral Research Panel with One Codex software, 12 Reactions, Kit
  • 103548 Twist Comprehensive Viral Research Panel with One Codex software, 96 Reactions, Kit

NGS Library Preparation Kits

Enzymatic Fragmentation 2.0

Mechanical Fragmentation With Amplification Mix

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② Universal Adapter System


NGS_reagents

① Fast Hybridization and Wash Kit

  • 15分鐘即可完成雜合反應
  • 建庫製備僅需5-8小時,單日無縫接軌後續實驗
  • 一天內從樣本製備到定序上機,大幅降低人力成本
  • 可微調反應條件符合各項樣品建庫需求
  • 彈性調整雜合時間以利最佳數據產出
  • 維持高品質高精準度樣本偵測率

ngs_fast-hybridization-and-wash-kit

      Product list

  • 101278 Twist Fast Hybridization and Wash Kit, 2 Reactions
  • 104180 Twist Fast Hybridization and Wash Kit with Amplification Mix, 12 Reactions
  • 101174 Twist Fast Hybridization and Wash Kit, 12 Reactions
  • 104181 Twist Fast Hybridization and Wash Kit with Amplification Mix, 96 Reactions
  • 101175 Twist Fast Hybridization and Wash Kit, 96 Reaction

① NGS Methylation Detection System

  • 低濃度DNA (10 – 200 ng) 檢測進量及低DNA損害流程,保護您珍貴的樣本
  • CpGs偵測率高( > 15%)
  • 先進酵素法轉換無甲基化cytosines降低偽陽性
  • Twist獨家客製化探針及試劑精準抓取目標
  • 適用於高複雜性文庫及CG 比率不均的樣本
  • 獨家Methylation Enhancer提升甲基化偵測率
  • 4小時即可完成雜合反應,亦可自由調整時間最佳化實驗結果
  • 智能設計、精準合成、品質控管提高探針偵測率
  • 獨家探針設計可同時抓取雙股、甲基化與無甲基化DNA
  • 適用於各種Panel大小、目標區域偵測及多種樣本混合等需求

ngs_ngs-methylation-detection-system ngs_ngs-methylation-detection-system_2

      Product list

  • 101976 NEBNext® EM-seq™ Kit for Twist Targeted Methylation Sequencing, 96 Samples
  • 103557 Twist Methylation Enhancer, 12 Reactions
  • 100578 Twist Universal Blocker, 12 Reactions
  • 100983 Twist Binding and Purification Beads Kit, 12 Reactions
  • 101174 Twist Fast Hybridization and Wash Kit, 12 Reactions
  • 103496 Twist Targeted Methylation Sequencing Workflow, 96×12 Reactions (contains all reagents listed above)

NGS_custom

① Twist Custom Panels

  • 獨家探針設計並優化
  • 可客製化目標序列覆蓋率
  • 精準且快速生產
  • NGS驗證均一度、再現性及濃度

 

NGS_custom_2

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